Histamine release in mast cells: Intracellular Ca-mobilization requires ATP and is modulated by pH7 tryptase
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چکیده
منابع مشابه
Inhibition of tryptase release from human colon mast cells by histamine receptor antagonists.
The main objective of this study was to investigate the ability of histamine receptor antagonists to modulate tryptase release from human colon mast cells induced by histamine. Enzymatically dispersed cells from human colon were challenged with histamine in the absence or presence of the histamine receptor antagonists, and the tryptase release was determined. It was found that histamine induced...
متن کاملMechanisms of nonimmunological histamine and tryptase release from human cutaneous mast cells.
BACKGROUND If mast cells are stimulated they release multiple mediators that delineate markers for immunologic and nonimmunologic reactions; histamine and tryptase are the two best known. Although histamine can be assayed in plasma, it is a nonspecific marker with a very short half-life. Tryptase has a longer half-life, but its release has not been proven to be specific for anaphylaxis. The aut...
متن کاملA role for tryptase in the activation of human mast cells: modulation of histamine release by tryptase and inhibitors of tryptase.
Tryptase, the most abundant protein product of human mast cells is emerging as an important mediator and target for therapeutic intervention in allergic disease. We have investigated the potential of tryptase and inhibitors of tryptase to modulate histamine release from human mast cells. Addition of purified human tryptase in concentrations ranging from 1 to 100 mU/ml stimulated a concentration...
متن کاملInduction of tryptase and histamine release from human colon mast cells by IgE dependent or independent mechanisms.
AIM To investigate the tryptase and histamine release ability of human colon mast cells upon IgE dependent or independent activation and the potential mechanisms. METHODS Enzymatically dispersed cells from human colons were challenged with anti-IgE or calcium ionophore A23187, and the cell supernatants after challenge were collected. Both concentration dependent and time course studies with a...
متن کاملEffects of C18 Fatty Acids on Intracellular Ca2+ Mobilization and Histamine Release in RBL-2H3 Cells
To investigate the underlying mechanisms of C18 fatty acids (stearic acid, oleic acid, linoleic acid and α-linolenic acid) on mast cells, we measured the effect of C18 fatty acids on intracellular Ca(2+) mobilization and histamine release in RBL-2H3 mast cells. Stearic acid rapidly increased initial peak of intracellular Ca(2+) mobilization, whereas linoleic acid and α-linolenic acid gradually ...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1990
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)55310-0